FIG 3.
ΔflcC mutant cells show defects in the collar structure and have fewer stator units assembled in the motor. (A and B) A central section of the subtomogram average (16-fold symmetrized) of WT and ΔflcC flagellar motors, respectively. The top portion of the collar (indicated by a white arrow) is absent from the ΔflcC motor. (C and D) Class averages of the collar region (dashed box in panel B) with and without the density of the stator complex in the ΔflcC motor, respectively. (E and F) A tilted side view of the 3D rendering of WT and ΔflcC flagellar motors (with stator complexes), respectively. (G) A histogram showing stator occupancy in the WT, ΔflcB, and ΔflcC motors, respectively. Refer to Materials and Methods section for details about the calculation of stator occupancy.