FIG 2.

Roles of segment length and direct repeat sequences in DelVG deletion formation. (A) Observed normalized junction counts per segment from intracellular DelVGs isolated at 6 hpi were compared to junction counts predicted by a model (expected) that assumes a simple positive correlation between segment length and DelVG junction count (see Materials and Methods). (B) Numbers of intracellular DelVG junctions detected at 6 hpi with no sequence repetition flanking the deletion breakpoints (direct repeat length = 0) or with repeated sequences of various length (direct repeat length = 1 to 9) for the indicated segments (observed). Expected values plot the numbers of junctions with the indicated repeat lengths predicted from model simulations in which junction formation is random. Junction counts are plotted as a percentage of the total number of DelVGs detected for a given segment. The data are presented as means (n = 3 cell culture wells) ± the standard deviations. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant (two-way ANOVA).