FIG 5.

Targeting sphingolipid and fatty acid desaturation pathways for drug development. (A) MICs of 10-thiastearic acid, thiocarlide, and myriocin. The MICs were obtained from 5 independent experiments with 4 replicates each. (B) Effect of fatty acid desaturase inhibitors on the fatty acid profile. Cells were grown in half of each compound MIC and submitted to fatty acid analysis. The values represent the ratio of peak areas of unsaturated to saturated fatty acids. (C) Effect of myriocin on the lipidomics profile. Cells were grown in half the myriocin MIC and submitted to fatty acid analysis. The values represent the mass spectral signal intensity on the apex of the peak. *, P ≤ 0.05 by Student's t test when comparing the control and treatments.