FIG 6.

Toxicity and efficacy of lipid metabolism inhibitors against intracellular infection of AMJ2 alveolar macrophages with Histoplasma capsulatum. (A) Viability of AMJ2 alveolar macrophages, measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, after exposed to different concentrations of myriocin, 10-thiastearic acid, and thiocarlide. (B and C) Intracellular killing of H. capsulatum by AMJ2 alveolar macrophages treated with the low and high concentrations of myriocin, 10-thiastearic acid (10-TA), and thiocarlide. The concentrations are highlighted in panel A. Bars represent the combination of 4 independent experiments with 3 replicates each (n = 12). *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001 by one-way ANOVA followed by Dunnett’s test comparing the control and treatments.