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. 2021 Nov 23;12(6):e02893-21. doi: 10.1128/mBio.02893-21

FIG 2.

FIG 2

Pyocin gene upregulation and ciprofloxacin sensitivity in Δ69700 is caused by deficiency of xerC. (A) Schematic of the lppL operon showing the encoded enzymes and their relative expression (fold change) in the ΔamrZ Δ69700 strain (MTC1398) versus the ΔamrZ strain (MTC590). (B) Representative growth curves of PA14 (MTC1) and the ΔxerC (MTC2266) and ΔxerC attB::CTX-1-PlppL-xerC (“attB::xerC”) (MTC2262) mutants in LB-Lennox medium with and without 0.03 μg/mL ciprofloxacin. (C) Representative growth curves of PA14 and the Δ69700 (MTC1513) and Δ69700 attB::CTX-1-PlppL-xerC (“attB::xerC”) (MTC2264) strains in LB-Lennox medium with and without 0.03 μg/mL ciprofloxacin. Error bars in panels B and C show standard deviations for at least three technical replicates. (D) Indicator assays showing R/F pyocin production by PA14 compared to Δ69700 and ΔxerC strains complemented or not with xerC (attB::CTX-1-PlppL-xerC). The supernatants were diluted 2-fold with fresh sterile LB where indicated.