FIG 6.
Effect of XerC recombinase inactivation. (A) Sequence alignment of the C-terminal ends of E. coli MG1655 and P. aeruginosa PA14 XerC proteins showing conservation (cyan boxes) and the catalytic Tyr residue (yellow). (B) Representative phase-contrast and fluorescence micrographs of PA14 xerCY272F bearing a P07990-gfp reporter at attB (MTC2341). (C) Line histograms of GFP mean fluorescence in individual PA14 xerCY272F cells (MTC2341). (D) Representative transcriptional profiles (P07990-lux reporter) of PA14 xerCY272F cells (MTC2339). Error bars show standard deviations for three technical replicates; some error bars are smaller than the symbols.