TABLE 1.
List of primers used in this study
| Name | Purposea | Sequence (5′→3′) | Reference |
|---|---|---|---|
| Gib_CF1 | pCF203 | ATAAAATGCTTGGGAGATGACGCCCGCCCCAC | This study |
| Gib_CF2 | pCF203 | ATAATCTCCTCTATTTCCATGAGAATCACTCCTATTTTTTTGATATATACATCATAACATTAC | This study |
| Gib_CF3 | pCF203 | AAAAAATAGGAGTGATTCTCATGGAAATAGAGGAGATTATAGAGAAAGTTGCTAGG | This study |
| Gib_CF4 | pCF203 | TGCGGGTCGTGGGGCGGGCGTCATCTCCCAAGCATTTTATGAGCCCTAGC | This study |
| Gib_CF5 | pSB1 | GCCGGTGGTTACCGTGATATTATCTATTACTATATCCCTATATAAAGAATACTCAAAAAATGGGC | This study |
| Gib_CF6 | pSB1 | GTAATAGATAATATCACGGTAACCACCGGCTAGCAGGTGATGCATATGGCTAAAATGAGAATATCAC | This study |
| Gib_CF7 | pSV1_1 | TATTTTGAATCCATTGCGTTGCGCTCACTG | This study |
| Gib_CF8 | pSV1_1 | TGGGCGGCCGGCCGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAG | This study |
| Gib_CF9 | pSV1_1 | AAATATTAACGCGGCCGGCCGCCC | This study |
| Gib_CF10 | pSV1_1 | TGCATTTTTTTGCGGCGCGCCCTGACA | This study |
| Gib_CF11 | pSV1_1 | AGCGCAACGCAATGGATTCAAAATAGATTCATAATGGAGTCATCCACG | This study |
| Gib_CF12 | pSV1_1 | TCAGGGCGCGCCGCAAAAAAATGCAAATAAAATTTGGGGTGG | This study |
| Res_CF1 | pSV1_2 | CGTACTGCAGCGATCGCGGTCATATGGATACAGCGGCC | This study |
| Res_CF2 | pSV1_2 | GTTATGGATTATAAGCGGCCGGC | This study |
| Gib_CF13 | pMVS1111A:Psynth-bgaB | CCACCCTGCCACCCCAAATTTTATTTGCATTTTTTTGCGGGTTAATTAAGCCTGGAGGAATGCCTTTATATAGG | This study |
| Gib_CF14 | pMVS1111A:Psynth-bgaB | TTTATATATTTTTAATTCACTGGGGGCAATTCTGTCAGGGCGCGCCTGGGGTCGTGCGCTC | This study |
| Gib_CF15 | pMVS1111A:PhmtB/Pmrt(M.t.)-bgaB | CGGCTCTAGCTATGTCCGATC | This study |
| Gib_CF16 | pMVS1111A:PhmtB/Pmrt(M.t.)-bgaB | CACTGGGGGCAATTCTGTCAG | This study |
| Gib_CF21 | pCF201 | AATACAAGAAAGGCGCGCCAAATCATTATATAGGACCTTGATAAAATTTTTTAGAGGGC | This study |
| Gib_CF22 | pCF201 | ACCTGACGTGTGGCCGGCCCGATTCAAATATAACAGCCGTTATAACACCGC | This study |
| Gib_CF23 | pCF201 | AATCGGGCCGGCCACACGTCAGGTGGCACTTTTCG | This study |
| Gib_CF24 | pCF201 | CATCCACGGATGCGATCGCCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGC | This study |
| Gib_CF17 | pCF202 | AAAAAATAGGAGTGATTCTCATGGCTAAAATGAGAATATCACCGGAAT | This study |
| Gib_CF18 | pCF202 | TGCGGGTCGTGGGGCGGGCGCTAAAACAATTCATCCAGTAAAATATAATATTTTATTTTCTCCCAAT | This study |
| Gib_CF19 | pCF202 | GATATTCTCATTTTAGCCATGAGAATCACTCCTATTTTTTTGATATATACATCATAACATTAC | This study |
| Gib_CF20 | pCF202 | TACTGGATGAATTGTTTTAGCGCCCGCCCCACG | This study |
| Res_LM1 | pMVS1111A:PhmtB-fdhZ-245 | ATCGGCTTAGGCGCGCCTGCTCATCGTCAATTCTAGTAGAGTCATGAATCATTATGCAGG | This study |
| Res_LM2 | pMVS1111A:PhmtB-fdhZ-245 | GCTTAGCGCATTAATTAACCGCCCATTTTTTGAGTATTC | This study |
| Gib_LM1 | pLM201 | TAACAGCGGCGCTATCAAGGTCCTGCATAATGATTCATGACGCCCGCCCCACG | This study |
| Gib_LM2 | pLM201 | TTTGCCGTATCTGCAGGCGATTTAAAAGATGATCCCATGAGAATCACTCCTATTTTTTTG | This study |
| Gib_LM3 | pLM201 | TTATGATGTATATATCAAAAAAATAGGAGTGATTCTCATGGGATCATCTTTTAAATCGCC | This study |
| Gib_LM4 | pLM201 | TCCTTTCGGTCGGGCGCTGCGGGTCGTGGGGCGGGCGTCATGAATCATTATGCAGGACC | This study |
| Gib_LM5 | pLM202 | ATCCTATATAAATATATCGCTAATTTTAAGGTTTTTCTGAGCCATCGGTTGGTTCATGGGTTAATTAAGAATACTCAAAAAATGGGCG | This study |
| Gib_LM6 | pLM202 | CGATATATTTATATAGGATTATATGAATAGATAATATCACATAAAATGAGGTGGTTAATTATGGGATCATCTTTTAAATCGCCTGCAG | This study |
| Seq_CF1 | Specific for gDNA M. t.1.5 kb | CCACCAGTTCGACTCCCTGG | This study |
| Seq_CF2 | Specific for gDNA M. t.1.5 kb | CTGTTAAAGGCGGGGGTGG | This study |
| Seq_CF3 | Specific for gDNA M. t. 2.8 kb | CTTGGGTGATGATGGGATGTATTG | This study |
| Seq_CF4 | Specific for gDNA M. t. 2.8 kb | CGAGGAGAAACACATCCAGCTG | This study |
| Seq_CF5 | Specific for pME2001 replicon | GTTAATCCAGCACATCCTCC | This study |
| Seq_CF6 | Specific for pME2001 replicon | CCTGTCCAACTTATACCTTTGG | This study |
| Seq_CF7 | Analysis of bgaB constructs | CCCCATAACATCGGCACAGTAC | This study |
| Seq_CF8 | Analysis of bgaB constructs | CCTGGCTGGGGTTAATAAATGTTG | This study |
| Seq_LM1 | Analysis of fdhZ-245 constructs | GATTTCTGGAATCCGCCATGGG | This study |
| Seq_LM2 | Analysis of fdhZ-245 constructs | CTAATAGTCGCCGATCCAAG | This study |
| Seq_LM3 | Analysis of fdhZ-245 constructs | GGTTCCTGGCTTGAATG | This study |
| Seq_LM4 | Analysis of fdhZ-245 constructs | GAGAAGCAAAGGATGACTG | This study |
| Seq_LM5 | Analysis of fdhZ-245 constructs | CAGCACCCATCTTATTCG | This study |
| Seq_LM6 | Analysis of fdhZ-245 constructs | GCAGTTAAGAAGGGTTCG | This study |
| Seq_LM7 | Analysis of fdhZ-245 constructs | GGCTCCGTTATAAGGGTTG | This study |
| Seq_LM8 | Analysis of fdhZ-245 constructs | CTGAATGGATCGAGAAAGG | This study |
| Seq_LM9 | Analysis of fdhZ-245 constructs | CATTCTTTCGAGATGGAAG | This study |
| Seq_LM10 | Analysis of fdhZ-245 constructs | CCTATATTCGCATTCGTGG | This study |
| Seq_LM11 | Analysis of fdhZ-245 constructs | ATGTTTGCCACACTGTG | This study |
| Seq_LM12 | Analysis of fdhZ-245 constructs | GGTGGGGTTTTGGTGTGCG | This study |
a
Abbreviations: gDNA, genomic DNA; M. t., M. thermautotrophicus.