TABLE 3.
List of plasmids used in this study
| Name | Function | Reference | M. thermautotrophicus straina |
|---|---|---|---|
| pMTL83151 | Shuttle vector for Clostridia spp. | Heap et al. (27) | - |
| pMU131 | Shuttle vector for Thermoanaerobacter spp. | Shaw et al. (23) | - |
| pME2001 | Cryptic plasmid of M. marburgensis | Bokranz et al. (50) | - |
| pBBR1-MCS2 | Standard cloning vector in E. coli | Kovach et al. (51) | - |
| pUC19 | Standard cloning vector in E. coli | Yanisch-Perron et al. (52) | - |
| pYS3 | Shuttle vector for Pyrococcus furiosus including Simr | Waege et al. (21) | - |
| pME2508 | PeiP production in E. coli | Luo et al. (48) | - |
| pCF200 | pUC57 vector including synthesized PmcrB(M.v.)_Purr codon-optimized for M. thermautotrophicus, Tmcr | This study | - |
| pCF201 | pUC19 vector including native M. thermautotrophicus Z-245 fdhZ-245 operon with putative promoter region | This study | - |
| pLM201 | Exchange of Neor to coding region of fdhZ-245 from pCF201 in pCF204 | This study | - |
| pLM202 | Exchange of PmcrB(M.v.) to PhmtB in pLM201 | This study | - |
| pCF203 | Exchange of Purr to Simr in pCF200 | This study | - |
| pCF204 | Exchange of Purr to Neor in pCF200 | This study | - |
| pCF404 | pUC57 including 1 kb up- and downstream of annotated pyrF gene (MTH_RS00570) and PmcrB(M.v.)_Purr | This study | - |
| pCF407 | Exchange of PmcrB(M.v.)_Purr to PmcrB(M.v.)_Neor in pCF404 | This study | - |
| pSB1 | Exchange of PmcrB(M.v.) promoter to Psynth in pCF407 | This study | - |
| pSV1_1 | Shuttle vector construct containing PmcrB(M.v.)_Simr and pBBR1MCS2 backbone and pME2001 replicon | This study | - |
| pSV1_2 | Shuttle vector construct containing PmcrB(M.v.)_Simr and pMTL backbone and pME2001 replicon | This study | - |
| pSV1_3 | Shuttle vector construct containing PmcrB(M.v.)_Neor and pMTL80151 backbone and pME2001 replicon | This study | - |
| pMVS-V1 | Shuttle vector construct containing Psynth_Neor and pMTL backbone and pME2001 replicon | This study | x |
| pMVS1111A:Psynth-bgaB | Shuttle vector construct pMVS-V1 including β-galactosidase (bgaB) gene and promoter Psynth | This study | x |
| pMVS1111A:PhmtB-bgaB | Shuttle vector construct pMVS-V1 including β-galactosidase (bgaB) gene and promoter PhmtB | This study | x |
| pMVS1111A:Pmrt(M.t.)-bgaB | Shuttle vector construct pMVS-V1 including β-galactosidase (bgaB) gene and promoter Pmrt(M.t.) | This study | x |
| pMVS1111A:Psynth(BRE)-bgaB | Shuttle vector construct pMVS-V1 including β-galactosidase (bgaB) gene and promoter Psynth(BRE) | This study | x |
| pMVS1111A:PhmtB-fdhZ-245 | Shuttle vector construct pMVS-V1 including fdhZ-245 operon from M. thermautotrophicus Z-245 and promoter PhmtB | This study | x |
a
-, strain not available; x, strain available.