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. 2021 Nov 23;12(6):e03000-21. doi: 10.1128/mBio.03000-21

FIG 3.

FIG 3

M. circinelloides FKBP12 does not functionally complement A. fumigatus FKBP12. (A) Growth of the wild-type A. fumigatus strain (KU80) and the strain expressing the hFKBP12, McFKBP12, and McFKBP12-Y88F proteins in the absence and presence of FK506 for 5 days at 37°C. (B) Microscopic localization of the McFKBP12 and McFKBP12-Y88F proteins in vivo in the absence or presence of FK506. Arrowheads show nuclear localization of McFKBP12. Arrows indicate binding of McFKBP12 and McFKBP12-Y88F to A. fumigatus calcineurin at the hyphal septum. (C) McFKBP12(Y88F)-FK506 structure (cartoon representation) with the position of common H-bonds (line representation) noted with labeled residues (left). The right image shows the H-bonds to FK506 gained (cyan) or lost (purple) due to the Tyr88Phe mutation.