Fig. 3.

Intracellular delivery and gene silencing of GCE/siRNA in vitro. (A) Confocal images treated by PBS, naked siRNA, GCE/siRNA, and Lipo/siRNA; siRNA was labled with FAM fluorescent molecule (green) to show the amount of siRNA into cells, and Hoechst 33,342 (blue) for cell nucleus. (B) Survivin-mRNA levels of the MCF-7 cells treated with different survivin-siRNA formulations were tested by RT-PCR. (C) Survivin protein levels treated with different survivin-siRNA formulations were tested by ELISA assay. The cells with no treatment was considered as a control. The data were presented as the mean ± SD, n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)