FIGURE 2.

SPtA 9150 engages caspase‐4, ASC and the NLRP3 inflammasome to induce pyroptosis. (a) Representative immunoblots from THP1 cell lysates stably expressing non‐targeting (CTRL) or miRNA30E (miR) against caspase‐4 or ASC as labelled. (b) PI uptake into THP1 cells treated with vehicle (DMSO) or MCC950 and infected with SPtA 9150. (c) PI uptake into CTRL^miR or ASC^miR THP1 cells infected with SPtA 9150. (d) Immunoblots in THP1 cell lysates stably transduced with miRNA30E (miR) against GSDMD or a non‐targeting control (CTRL) as indicated. (e) PI uptake into the indicated THP1 cells infected with SPtA 9150. Mean ± SEM from 4 (b, c), or 3 (e) independent experiments are shown. * p < .05 by paired Student's t‐test (c–e) or the indicated comparisons by two‐way ANOVA (B; with correction for multiple comparisons); ns, not significant