Table 3.

Studies on the successful preparation of blood-compatible micelles.

Components or strategy	Preparation method	Route	Drug or purpose	Assessment format	Observations	Ref.
Self-assembled nano-colloidal polymeric micelles (LP-PMs) of Soluplus and PF127	Thin-film hydration method	i.v.	Lapatinib	Haemolysis Platelet aggregation test	The degree of hemolysis is within an acceptable range. No aggregation was observed in the control or LP-PM treated blood samples.	[76]
Amphiphilic maltodextrin-ursodeoxycholic acid (FA and lactobionic acid were coupled to the surface of micelles)	Dialysis method	–	Sulfasalazine and resveratrol	Serum stability Haemolysis	All the evaluated parameters are within the acceptable range.	[77]
Redox-sensitive heparin-β-sitosterol	Probe sonication and aqueous dialysis	i.v.	Doxorubicin	Haemolysis	The preparation has no significant effect on red blood cells.	[78]
A series of Chondroitin sulfate/poly (D,L-lactideco-glycolide) block copolymers (CHs-b-PLGA) with different length of hydrophobic block	Sonicate method	i.v.	Doxorubicin	Haemolysis	As the molecular weight of PLGA increases, the hemolysis rate of micelles increases slightly. However, the degree of hemolysis in all formulations is within an acceptable range.	[79]
Amphiphilic bifunctional pullulan derivative	Dialysis method	i.v.	Doxorubicin	Haemolysis	The degree of hemolysis is within an acceptable range.	[80]
Amphiphilic styrene-maleic acid (SMA) copolymer	Dialysis method	–	3,4-Difluorobenzylidene curcumin (CDF)	Serum stability	SMA-CDF nanomicelles exposed to fibrinogen or 50% FBS did not find any significant difference in particle size up to 72 h.	[81]
Poly(D-lactide), poly(L-lactide) and stereocomplex micelle	Nanoprecipitation method	i.v.	Doxorubicin	Serum stability Haemolysis	In PBS buffered BSA solution (30.0 mg/ml), all loaded micelles showed excellent stability during the 72-h measurement. The degree of hemolysis is within an acceptable range.	[82]
A copolymer composed of carboxybetaine methacrylate (CBMA) as hydrophilic segment and 2-(methacryloyloxy)ethyl lipoate (MAEL) as hydrophobic	Dialysis method	i.v.	Doxorubicin	Serum stability	No increase in the size of drug-loaded micelles was observed in 1 mg / mL PBS solution and 50% FBS at 37 °C.	[83]