Fig. 1.

Chemical genomic analysis of the P. insularum homogenate. (A) Bow-tie representation of molecular functions of the 23 genes buffering the cellular response to the P. insularum homogenate. The 23 genes were identified from the barcode-sequencing haploinsufficiency analysis of 6,000 heterozygous deletion strains using a statistical cutoff to detect significant differences in growth in the presence of the homogenate compared to the control (SI Appendix, Fig. S1). (B) GO enrichment analysis using YeastEnrichr distinguishes iron transport as being significantly over-represented within the 23 genes. The enrichment score is a combined score that integrates P and z-score of expected and observed classification of a gene set to molecular functions. Percent growth of (C) WT or (D) fet3Δ in increasing concentrations of P. insularum homogenate with the absence or addition of 100 µM FeCl₃, FeSO₄, or ZnCl₂ compared to growth in media without P. insularum homogenate. Data shown are average and SD of three independent experiments, each with three technical replicates.