(A) Design of the experiment for data in (B)–(E) in which all animals are given ad libitum access to oLFD. n = 5–6/group.
(B) Infused kcals and ad libitum kcals consumed.
(C) Average ad libitum intake during 11-kcals infusions with LFD or HFD.
(D) Body weight during infusions.
(E) Experimental design for (F) and (G) (n = 6).
(F) Infused and ad libitum caloric intake.
(G) Average intake of days 4–5 during iLFD compared with that of days 6–14 during iHFD. Data are displayed as mean ± standard deviation or as individual data points in (C). *p < 0.05, comparing iLFD diet group to iHFD using a Student’s t test except in (C) and (G) for which paired t tests were used for within-group comparisons. Unshaded bars show intake during iLFD while black bars show intake during iHFD.