Skip to main content
. 2021 Oct 14;16(11):2137–2143. doi: 10.1021/acschembio.1c00542

Figure 3.

Figure 3

TLC-MALDI performed with minimal peptide fragments reveals proximal PARP selectivity. (a) P14 was incubated with P14i1 in the presence of increasing concentrations of NAD+, and the resulting MS spectra were normalized and plotted against each other to visualize the increase in m/z due to ADPr (+541). The bar graphs depict the fraction of the total peptide that has been modified (mean ± S.E.M., n = 3). The sequence of the P14i1 peptide fragment is provided. (b) P15 is unable to transfer ADPr to the P14i1 peptide. Experiments were performed as in (a) in the presence of 1 mM NAD+. (c) Loss of the acidic modification site prevents ADP-ribosylation. Experiments were performed as in (a) in the presence of 1 mM NAD+. *represents p-value <0.05, two-tailed Student’s t test.