Skip to main content
. 2021 Nov 4;16(11):2130–2136. doi: 10.1021/acschembio.1c00538

Figure 4.

Figure 4

HMSiR-tubulin (3) performance in 2D-MINFLUX and 2D STED nanoscopy. (a) Confocal and 2D STED with 775 nm images of living mouse neurons stained with 300 nM HMSiR-tubulin (3). Scale bar 10 μm; inset 1 μm. Numbers represent fwhm. (b) MINFLUX image of microtubules in fixed U-2 OS cells stained with 20 nM HMSiR-tubulin (3) acquired in 3 h and rendered with 4 nm pixel size. Scale bar 500 nm. Line profile of the region (200 nm in width) shown in the inset. (c) Microtubule fwhm measured by SMLM and MINFLUX nanoscopy. MINFLUX data are from 5 fields of view, 2 independently prepared samples.