Fig. 3.

Inhibition of cell division attenuates the decline of MIR156C. (A and B) Low temperature reduces root growth rate (A) and attenuates the decline of MIR156C (B). The pMIR156C:GFP-N7 pAT2G18020:H2B-mCherry dual reporter seedlings were grown on a vertical 0.5× MS plate for 3 d (3 DAS) and then transferred to 4 °C for 2 or 4 d. (Scale bars, 0.5 cm in A and 50 µm in B). (C) Quantification of GFP-N7 mRNAs in the pMIR156C:GFP-N7 reporter at root distal regions in response to cold treatment by qRT-PCR. Expression of GFP-N7 before treatment (3 DAS) was set to 1.0. Plants were treated as described in A. Two biological replicates were performed. Error bars show the SEM. (D and E) FVP inhibits cell division in roots (D) and attenuates the decline in MIR156C (E). The pMIR156C:GFP-N7 pAT2G18020:H2B-mCherry dual reporter seedlings were grown on a vertical 0.5× MS plate for 3 d (3 DAS) and then treated with 10 μM FVP for 2 or 4 d. (Scale bars, 0.5 cm in D and 50 µm in E.) (F) Quantification of GFP-N7 mRNAs in the pMIR156C:GFP-N7 reporter at root distal regions in response to FVP treatment by qRT-PCR. The expression level of GFP-N7 before treatment (3 DAS) was set to 1.0. Plants were treated as described in D. Three biological replicates were performed. Error bars show the SEM. (G and H) Torin 2 inhibits cell division in roots (G) and attenuates the decline in MIR156C (H). The pMIR156C:GFP-N7 pAT2G18020:H2B-mCherry dual reporter seedlings were grown on a vertical 0.5× MS plate for 3 d (3 DAS) and then treated with Torin 2 for 2 or 4 d. (Scale bars, 0.5 cm in G and 50 µm in H.) (I) Quantification of GFP-N7 mRNAs in the pMIR156C:GFP-N7 reporter at root distal regions in response to Torin 2 treatment by qRT-PCR. The expression level of GFP-N7 before treatment (3 DAS) was set to 1.0. Plants were treated as described in G. Two biological replicates were performed. Error bars show the SEM.