Fig. 5.

Knockdown of Csi-let-7a-5p decreased inflammatory responses in macrophages by the SOCS1 and Dectin-1–mediated NF-κB signaling pathway. (A) Csi-let-7a-5p specific siRNA oligonucleotide or scrambled siRNA labeled with a green dye were observed within worm bodies. (B) The relative expression of mature Csi-let-7a-5p in the Csi-let-7a-5p-siRNA (inhibitor), scrambled siRNA, or 1640-treated worms (Left). CsEVs from these different treated worms were prepared as described in Fig. 1A; the relative expression of mature Csi-let-7a-5p in CsEVs (from 1640-treated worms), scrambled-CsEVs (from scrambled siRNA-treated worms), and inhibitor-CsEVs (from Csi-let-7a-5p-siRNA–treated worms) were determined by qPCR. (C) The relative expression of Socs1 and Clec7a in macrophages that were stimulated by inhibitor-CsEVs, scrambled-CsEVs, CsEVs, or PBS for 24 has been determined by qPCR. (D) Western-blot showed the protein levels of SOCS1, Dectin-1, and P-p65 in macrophages that were stimulated by PBS, CsEVs, scrambled-CsEVs, or inhibitor-CsEVs for 24 h. (E) The secretion of IL-6 and TNF-α in the cultures of macrophages that were stimulated by scrambled-CsEVs or inhibitor-CsEVs for 24 h as determined by ELISA. The values were expressed as mean ± SEM. Compared with indicated groups, *P < 0.05, **P < 0.01, ***P < 0.001.