Fig. 3.

Effect of SA187 inoculation in Arabidopsis on 1/2MS + 100 mM NaCl. Shoot and root sulfur levels of Arabidopsis grown under salt stress, in the presence or absence of SA187. Values represent means of three biological experiments, each in three technical replicates (n = 9). Error bars represent SE. Asterisks indicate the statistically significant difference based on the Mann–Whitney U test (***P < 0.001) when compared to mock plants (A). Total fresh weight beneficial index of 21-d-old plants treated with SA187, 100 nM L-methionine, 100 nM L-cysteine, or 1 mM MgSO₄, on 100 mM NaCl. Values represent means of 3 biological experiments, each in 10 technical replicates (n = 30). Error bars represent SE. Asterisks indicate the statistically significant difference based on the Mann–Whitney U test (*P < 0.05; **P < 0.01) when compared to mock plants (B). Total fresh weight beneficial index of noncolonized (gray) and SA187-colonized (green) 21-d-old wild-type, sultr1, apk1/2, apr2, fry1, sir-1, or amiR-LSUc plants, on 1/2MS + 100 mM NaCl. Values represent means of 3 biological experiments, each in 12 technical replicates (n = 36). Error bars represent SE. Asterisks indicate the statistically significant difference based on the Mann–Whitney U test (**P < 0.01; ***P < 0.001) when compared to noninoculated wild-type plants (C). Accumulation of superoxide radicals (visualized by nitroblue tetrazolium staining) in 0 (control) and 100 mM NaCl (salt stress), in wild-type and amiR-LSUc mutant plants (D).