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. Author manuscript; available in PMC: 2021 Nov 23.
Published in final edited form as: Dev Biol. 2018 Oct 1;448(2):136–146. doi: 10.1016/j.ydbio.2018.09.021

Fig. 2. Wnt and FGF signaling regulate posterior-enriched notochord expression.

Fig. 2.

In situ hybridization against Brachyury (A-D), C11.331 (E-H), C12.115 (I-L) and C8.891 (M-P) on Hotta stage 23 embryos treated starting at mid gastrula (Hotta stage 12) with 1:1000 DMSO (A, E, I, M), 5 μM Wnt activator BIO (B, F, J, N), 4 μM FGF inhibitor U0126 (C, G, K, O) or BIO + U0126 (D, H, L, P). Representative images of the most common expression pattern for each gene/treatment are shown. Panels to the right of each image show the distribution of expression patterns observed. Scale bar = 50 microns.