KEY RESOURCES TABLE
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-mCherry | Biovision | Cat#5993 |
| Goat anti-rabbit polyclonal AlexaFluor488 | Invitrogen | Cat#A11034 |
| Goat anti-mouse polyclonal AlexaFluor488 | Invitrogen | Cat#A11029 |
| Goat anti-rabbit polyclonal AlexaFluor555 | Invitrogen | Cat#A22420 |
| Rabbit polyclonal anti-GFP | Invitrogen | Cat#A11122 |
| Mouse monoclonal anti-HA | Cell Signaling Technology | Cat#2367S |
| Bacterial and Virus Strains | ||
| n/a | ||
| Biological Samples | ||
| n/a | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| GSK inhibitor IX (BIO) | Sigma-Aldrich | Cat#361550; CAS667463-62-9 |
| U1026 | Sigma-Aldrich | Cat#662005; CAS109511-58-2 |
| SIGMA-FAST FastRed | Sigma-Aldrich | Cat#F4648 |
| AlexaFluor633 Phalloidin | Invitrogen | Cat#A22284 |
| Critical Commercial Assays | ||
| n/a | ||
| Deposited Data | ||
| Ciona SELEX data | ANISEED | https://www.aniseed.cnrs.fr/ |
| Experimental Models: Cell Lines | ||
| n/a | ||
| Experimental Models: Organisms/Strains | ||
| Wild Ciona robustus | M-REP, San Diego, CA | N/A |
| Oligonucleotides | ||
| See Table S1 | ||
| Recombinant DNA | ||
| Bra>hCD4:mCherry | Gline et al, 2015 | N/A |
| All C11.331>UNC76:Venus reporter plasmids | This paper | N/A |
| Bra>H2B:HA | This paper | N/A |
| Software and Algorithms | ||
| MATLAB | The Mathworks, Inc | Release 2012b |
| FIJI/ImageJ | Schindelin et al, 2012 | https://fiji.sc/ |
| LASAGNA-GRID | Lee & Huang, 2013 | (http://biogrid-lasagna.engr.uconn.edu/lasagna_search |
| Other | ||