Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 23;9:228. doi: 10.1186/s40168-021-01178-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — A. pulmonis is sufficient to elicit a strong inflammatory response in mice. a SPF C57BL/6 mice were treated with an antibiotic cocktail for 4 days. One day post antibiotics, the mice were daily orally colonized with bacterial cocktail (A. pulmonis, 5-Mix or residual 4-Mix, 10⁹ CFU/mouse/dose) or BHI until being euthanized. After 5 days bacterial colonization, the mice were exposed to 3% DSS for 7 days, followed by regular water for 3 days. b and c Changes of body weight (b) and disease activity index (DAI) (c) after 3% DSS administration. d–g Colon length (d), representative colons (e), representative colonic histological images (Scale bar = 40 μm and 10 μm) (f), colonic histological score (g) in mice treated with A. pulmonis, 5-mix bacterial cocktail, 4-mix bacterial cocktail and BHI. h SPF Il10^−/− mice were treated with antibiotics cocktail for 4 days. One day post antibiotics, the mice were daily orally colonized with bacterial cocktail (A. pulmonis or E. fergusonii, 10⁹ CFU/mouse/dose) or BHI for 3 weeks. i-l Colon length (i), representative colons (j), representative colonic histological images (Scale bar = 40 μm and 20 μm) (k), colonic histological score (l) from Il10^−/− mice treated with A. pulmonis, E. fergusonii and BHI. Results are shown as the mean± SEM. Each dot indicates an individual mouse. The statistical significance values are denoted as: *p < 0.05, **p < 0.01, *** p < 0.001, ****p < 0.0001. One-way ANOVA following Tukey’s multiple comparison test (d, g, j, and l); two-way ANOVA following Tukey’s multiple comparison test (b and c)