Skip to main content
. Author manuscript; available in PMC: 2021 Nov 23.
Published in final edited form as: Methods Appl Fluoresc. 2020 Feb 19;8(2):025004. doi: 10.1088/2050-6120/ab716a

Figure 2: Proof of concept FRAP experiment.

Figure 2:

(A, left) Fluorescence image of IRE1-GFP before bleaching. (A, second from left) Selected spot for bleaching IRE1-GFP with 488 nm light. (A, center) Fluorescence of IRE1-GFP during bleaching. (A, second from right) Fluorescence image of IRE1-GFP after bleaching of the selected spot (red outline). (A, right) Fluorescence image of IRE1-GFP after fluorescence recovery of the selected spot (red outline) (B) Normalized bleach-corrected intensity of the IRE1-GFP emission within the bleached region outlined in red. Scale bar: 1 µm.