FIG. 2.
Endothelialization of empty channels. (A) Whole-mount histology of engineered vessel in complete media (10 × magnification) and (B) section view of the vessel wall (60 × magnification). (C) Engineered vessel cultured in RF EGM-2 (10 × magnification). Inset shows partial delamination of vessel from vessel wall (dashed lines). (D) Quantification of engineered vessel diameter. (E) Sprouting behavior in engineered vessels in EGM-2 (20 × magnification). Inset shows tip-stalk phenotype of sprouts, with arrows indicating tip cells. (F) Sprout regression in RF EGM-2 (20 × magnification). (G, H) Quantification of sprouting behavior from engineered vessels. + EC (n = 14), − EC (n = 11), + ECR (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Color images are available online.