Table 1.
Antibody/fluorophore | Dilution | Catalog No.; company |
---|---|---|
Mouse monoclonal anti-vimentin | 1:200 | V6630-100 UL; MilliporeSigma |
Mouse monoclonal anti-collagen IV | 1:100 | SAB4200709-100 UL; MilliporeSigma |
Rabbit polyclonal anti-human VE-cadherin | 1:50 | 36-190-0; Fisher |
Bisbenzimide H 33342 trihydrochloride (Hoechst) | 1.5 μg/mL | B2261-100MG; MilliporeSigma |
Goat anti-mouse Alexa Fluor 488 | 1:400 | A-11001; Invitrogen |
Goat anti-rabbit Alexa Fluor 594 | 1:400 | A-11005; Invitrogen |
Griffonia Simplicifolia Isolectin B4, Dylight 594-labeled | 1:400 | DL-1207-.5; Vector Labs |