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. Author manuscript; available in PMC: 2021 Nov 23.
Published in final edited form as: Nat Protoc. 2021 May 5;16(6):2802–2825. doi: 10.1038/s41596-021-00517-1

Fig. 3 |. Typical result of a size selection gel for ribosome footprint samples digested with two different nucleases.

Fig. 3 |

MNase digestion results. Lanes 1 and 3: 20/100 ladder (IDT) with sizes of each band annotated to the left; lanes 2 and 4: two separate ribosome footprint samples. The size of ribosome footprint fragments usually ranges from 15 to 45 nt, as indicated by the bracket for the target area to cut.