Table 3 |.

Troubleshooting table

Step	Problem	Possible reason	Solution
22	No mitoribosome peak appears in UV absorption	Mitoribosomes exist at much lower abundance than cytosolic ribosomes. Sometimes the mitoribosome peak in UV absorption is not apparent	Collect the fractions between the 40S and 60S cytosolic ribosome subunit peaks and extract RNA from all of them, or check for mitochondrial ribosomes by western blot
74	No reverse transcription product observed in gel	Depending on sample input and technique during library preparation, the amount of sample can be very low at this stage. Nevertheless, even if a band is not visible, there might be enough cDNA to be amplified by PCR	Proceed by cutting the desired size range
87	No targeted band observed in PCR gel	Sample input at the start of the procedure might be too low, or the sample was lost during a previous step	If this continues to occur, we recommend increasing sample input and analyzing sample loss at each stage to identify the problem
93	Issues in mapping and computation	-	Refer to the GitHub page for some common problems in the bioinformatics analysis