| Reagent | Amount (μL) |
|---|---|
| 50% wt/vol PEG 8000 | 3.5 |
| 10× T4 RNA ligase 2 buffer | 0.5 |
| 20 μM Universal miRNA cloning linker | 0.5 |
| T4 RNA ligase 2, truncated K227Q | 0.5 |
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