| Reagent | Amount (μL) | Final concentration |
|---|---|---|
| Sample | 15 | - |
| 10× CircLigase buffer | 2 | 1× |
| 1 mM ATP | 1 | 50 μM |
| 50 mM MnCl2 | 1 | 2.5 mM |
| CircLigase (100 U/μL) | 1 | 5 U/μL |
| Total | 20 |
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