| Reagent | Amount (μL) | Final concentration |
|---|---|---|
| 2× Phusion high-fidelity PCR master mix with HF buffer | 40 | 1× |
| 10 μM Forward library PCR primer | 4 | 0.5 μM |
| 10 μM Indexed reverse library PCR primer | 4 | 0.5 μM |
| Nuclease-free H2O | 27 | - |
| Total | 75 |
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