| No | Step | Temperature | Duration |
|---|---|---|---|
| 1 | Initial denaturation | 98 °C | 1 min |
| 2 | Denature | 98 °C | 20 s |
| 3 | Annealing | 60 °C | 20 s |
| 4 | Extension | 72 °C | 10 s |
| 5 | Final extension | 72 °C | 3 min |
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