Table 1 |.

Comparison of different mitochondrial ribosome profiling methods

Reference	Species	Antibiotic pretreatmen	Ribosome isolation method	Ribonuclease	Fragment size selected (nt)	rRNA extraction	A-site alignment method	Most common footprint size (nt)
Standard ribosome profiling: McClincy and Ingolia33	Yeast	None	Sucrose cushion	RNase I	15–45, canonical footprint is ~28	Ribo-Zero Gold (Illumina)	5’-end offset from start codon for each read length	28
This paper	Human	None	Sucrose gradient, real-time collection with UV signal	MNase	15–45	None	3’-end offset from start codon	30
Rooijers et al.35 and Gao et al.67	Human	Yes	Sucrose gradient, mitoribosome identification with western blot	RNase I	25–36	None	5’-end offset by 16 nt	33
Couvillion et al.37 and Couvillion & Churchman36	Yeast	None	Immunoprecipitation using FLAG-tagged mitoribosome subunit	RNase I	36–42	Ribo-Zero Gold (Illumina)	3’-end offset from start codon	39
Pearce et al.38	Human	Yes	Sucrose gradient, mitoribosome identification with western blot	RNase I	25–35	Duplex strand nuclease treatment at amplicon stage	5’-offset using lysine stalling in ΔFLP cells	33