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. 2021 Sep 24;18(4):1021–1039. doi: 10.20892/j.issn.2095-3941.2020.0791

Figure 5.

Figure 5

Trajectory analysis reveals the developmental pathway of cytokine-trained NK cells. (A) Analysis of day 7 NK cells with Monocle2 pseudotime trajectory analysis to simulate the ontology (left) and positions of cells in all 4 clusters (right). (B) Individual clusters of day 7 NK cells are demonstrated in the trajectory. (C) Heatmap of selected gene expression profiles along the pseudotime trajectory. Group 1 genes, including B3GAT1, ITGA5 (CD49e), and KLRK1 (NKG2D), were more highly expressed at the late developmental stage. Group 2 genes, including PRF1, FCGR3A (CD16), and KLRF1 (NKp80), were more highly expressed at the late-middle stage. Group 3 genes, including SELL (CD62 L) and IL7R, were more highly expressed at the early-middle stage of development. Group 4 genes were expressed at an early stage of the developmental pathway, which was cluster #6. The group 5 gene GZMB showed higher expression at the initial and late developmental stages, and the group 6 gene ITGAM (CD11b) showed higher expression at the middle and late stages of development.