Figure 7.

Inhibition of EZH2 represses the formation of cytokine-induced trained NK cells and cell cycle progression. (A) PBMCs were labeled with CTV and cultured in vitro under control or preactivation conditions with or without UNC1999 treatment for 1 week, and then the cells were restimulated with IL-12/18. NK cells were analyzed for IFN-γ expression. (B) Representative flow cytometry plot of IFN-γ⁺ NK cells in the control and preactivated groups cultured with or without UNC1999 (left). A summary bar graph shows the percentage of IFN-γ⁺ NK cells in each indicated treatment group (right, n = 5). (C) Combined flow cytometry t-SNE plot of the CTV dilution and expression of EZH2 and Ki67 in NK cells of each indicated treatment group (n = 3). (D) Overlaid histograms show the CTV dye dilution profiles of samples analyzed in the CTV section of (C) (n = 3).