|
Antibodies
|
| Anti-GluR1-NT (N-terminus) Antibody, clone RH95 |
Merck Millipore |
Cat#MAB 2263; |
| Anti-GluR2 Antibody, clone 14C12.2 |
Merck Millipore |
Cat#MABN1189; |
| Anti-PSD95 antibody |
Abcam |
Cat#ab12093; |
| eIF4E (C46H6) Rabbit mAb |
Cell Signaling Technology |
Cat#2067; |
| p70 S6 kinase (49D7) Rabbit mAb |
Cell Signaling Technology |
Cat#2708 |
| Proteasome 19S Rpt1/S7 subunit monoclonal antibody (MSS1-104) |
Enzo Life Sciences |
Cat#BML-PW8825; |
| Proteasome 19S ATPase subunit Rpt6 monoclonal antibody (p45-110) |
Enzo Life Sciences |
Cat#BML-PW9265; |
| Proteasome 19S Rpt3/S6b subunit polyclonal antibody |
Enzo Life Sciences |
Cat#BML-PW8250; |
| Proteasome 20S core subunits polyclonal antibody |
Enzo Life Sciences |
Cat#BML-PW8155; |
| Purified anti-HA.11 Epitope Tag Antibody |
BioLegend |
Cat#901501; |
| Anti-Hsp72 Antibody, clone 3G7 |
Merck Millipore |
Cat#MABE973; |
| Anti-pan Ago Antibody, clone 2A8 |
Merck Millipore |
Cat#MABE56; |
| eEF2 antibody |
Cell Signaling Technology |
Cat#2332; |
| Phospho-p70 S6 kinase (Thr389) (1A5) |
Cell Signaling Technology |
Cat#9206; |
| Anti-TRIM32 antibody |
Abcam |
Cat#ab96612; |
| MOV10 Antibody |
Bethyl Lab |
Cat#A301-571A; |
| Monoclonal Anti-β-Tubulin III (neuronal) antibody (Tuj1) |
Sigma Aldrich (Merck) |
Cat#T8578; |
| GAPDH |
Sigma Aldrich (Merck) |
Cat#G9545; |
| Anti-HA.11 Epitope Tag Affinity Matrix |
BioLegend |
Cat#900801; |
| Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific (Invitrogen) |
Cat#A11055; |
| Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor 633 |
Thermo Fisher Scientific (Invitrogen) |
Cat#A21082; |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 546 |
Thermo Fisher Scientific (Invitrogen) |
Cat#A11030; |
| Goat anti-Rat IgG (H+L) Secondary Antibody, HRP |
Invitrogen |
Cat#31470; |
| Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) |
Jackson ImmunoResearch |
Cat#115-035-003; |
| Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) |
Jackson ImmunoResearch |
Cat#111-035-003; |
| AffiniPure Goat Anti-Mouse IgG (H+L) |
Jackson ImmunoResearch |
Cat#115-005-062; |
| Rabbit IgG Isotype Control |
Invitrogen |
Cat#10500C; |
| FK1 antibody |
Enzo Life Sciences |
Cat# BML-PW8805 |
| 20S Proteasome core subunits |
Enzo Life Sciences |
Cat# BML-PW8155 |
| Anti-pan Ago Antibody, clone 2A8 |
Millipore |
Cat# MABE56 |
| Anti-Dicer clone N167/7 |
Neuromab |
Cat# 75–196 |
|
Chemicals, Peptides, and Recombinant Proteins
|
| (−)-Bicuculline methochloride |
Tocris Bioscience |
Cat#0131; |
| Lactacystin |
AG Scientific |
Cat#SKU L-1147; |
| Tetrodotoxin |
Abcam |
Cat#ab120054; |
| Glutamate Receptor Endocytosis Inhibitor, GluR23y, YKEGYNVYG |
AnaSpec |
Cat#AS-62547; |
| Anisomycin from Streptomyces griseolus
|
Sigma-Aldrich |
Cat#A-9789; |
| Rapamycin from Streptomyces hygroscopius
|
Sigma-Aldrich |
Cat#R-0395; |
| Recombinant Protein G Agarose |
Invitrogen |
Cat# 15920010 |
| EDTA-free Protease Inhibitor Cocktail |
Roche (Sigma Aldrich) |
Cat#05892791001 |
| LY2584702 tosylate |
Sigma |
Cat# SML2892 |
| Cycloheximide |
Sigma Aldrich |
Cat# C1988; |
| SUPERaseIn RNase Inhibitor (20 U/μL) |
Invitrogen (Ambion) |
Cat# AM2694 |
| Phospahatase Inhibitor Cocktail 1 |
Sigma Aldrich |
Cat# P2850 |
| RNase A |
Thermo Fisher Scientific (Ambion) |
Cat# AM2269 |
| Rnase T1 |
Thermo Fisher Scientific (Ambion) |
Cat# AM2283 |
|
Critical Commercial Assays
|
| BCA protein assay kit |
Thermo Fisher Scientific (Pierce) |
Cat#23227; |
| Clean-Blot IP Detection Kit (HRP) |
Thermo Fisher Scientific (Pierce) |
Cat#21232; |
| Immobilon western chemiluminiscent HRP substrate |
Millipore |
Cat#WBKLS0500 |
| 20S Proteasome Assay Kit |
Enzo Life Sciences |
Cat# BML-AK740 |
| Dual luciferase Assay Kit |
Promega |
Cat# E1910 |
| SuperScript III First-Strand Synthesis System |
Thermo Fisher Scientific |
Cat# 18080051 |
|
Experimental Models: Cell Lines
|
| HEK293T |
ATCC |
ATCC #CRL-3216 |
|
Experimental Models: Organisms/Strains
|
| Sprague Dawley Rats |
National Brain Research Centre, India |
N/A |
| Ribo Tag transgenic mice in C57BL6/J background |
The Jackson Laboratory |
029977 |
| T29-1 CamK2α-Cre transgenic mice in C57BL6/J background |
The Jackson Laboratory |
005359 |
|
Recombinant DNA
|
| pLVTHM (used for preparing lentivirus) |
Addgene |
Plasmid #12247 |
| psPAX2 |
Addgene |
Plasmid #12260 |
| pMD2.G |
Addgene |
Plasmid #12259 |
|
Software and Algorithms
|
| ImageJ |
NIH |
RRID: SCR_003070;https://imagej.net/
|
| GraphPad Prism8 |
GraphPad Software |
RRID: SCR_002798; http://www.graphpad.com/
|
| MATLAB |
The MathWorks |
RRID: SCR_001622;http://www.mathworks.com/products/matlab/
|
| pCLAMP10.5 |
Molecular Devices |
RRID: SCR_011323;http://www.moleculardevices.com/products/software/pclamp.html
|
