Table 1.
Primers used for PCR amplifications.
| Gene | Protein | Primer sequence (5′ – 3′) | |
| P2RX7 | P2 × 7 | Forward: | TTCAGGCAGGCAGTATCACTC |
| Reverse: | CCACGGGAAAGACACAGGTAG | ||
| CASP11 | Casp-11 | Forward: | ATGGCTGAAAACAAACACCCT |
| Reverse: | TCAGTTGCCAGGAAAGAGGTAG | ||
| CASP1 | Casp-1 | Forward: | GGAAGCAATTTATCAACTCAGTG |
| Reverse: | GCCTTGTCCATAGCAGTAATG | ||
| IL1B | Il-1β | Forward: | TTCAGGCAGGCAGTATCACTC |
| Reverse: | CCACGGGAAAGACACAGGTAG | ||
| TNF | Tnf-α | Forward: | TTCAGGCAGGCAGTATCACTC |
| Reverse: | CCACGGGAAAGACACAGGTAG | ||
| NLRP3 | Nlrp-3 | Forward: | GCT CAG CTC TGA CCT CT |
| Reverse: | AGG TGA GGC TGC AGT TGT CT | ||
| TNFRSF11A | Rank | Forward: | TTAAGCCAGTGCTTCACGGG |
| Reverse: | ACGTAGACCACGATGATGTCGC | ||
| TNFSF11 | Rankl | Forward: | CAGAAGATGGCACTCACTGCA |
| Reverse: | CACCATCGCTTTCTCTGCTCT | ||
| Fusobacterium nucleatum (Target Species) | Foward: | CTT AGG AAT GAG ACA GAG ATG | |
| Reverse: | TGA TGG TAA CAT ACG AAA GG | ||
| Porphyromonas gingivalis (Target Species) | Foward: | CGCAGACGACAGAGAAGACA | |
| Reverse: | ACGGACAACCTGTTTTTGATAATCCT | ||
| ACT | β-actin | Forward: | TAT GCC AAC ACA GTG CTG TCT GG |
| Reverse: | TAC TCC TGC TTG CTG ATC CAC AT |