FIG. 1.
GST-p12DOC-1 associates with CDK2 in cell lysates. (A) CDK2 immunoblot showing cellular CDK2 from HeLa, 293, and A431 cells associating with the GST-p12DOC-1 fusion protein. Lanes 1, 4, and 7, GST control; lanes 2, 5, and 8, GST-p12DOC-1; lanes 3, 6, and 9, input lysate at 0.1×. (B) CDK2 immunoblot showing that GST-p12DOC-1 associates with the 34-kDa forms of CDK2. Lane 1, GST control; lanes 2 and 3, GST-p12DOC-1 (1 and 2 μg, respectively); lane 4, immunoprecipitation of CDK2. (C) Phosphotyrosine immunoblot showing that the p34CDK2 that associates with GST-p12DOC-1 does not contain phosphotyrosine residues (4G10; Upstate Biotechnology). The samples for panels B and C were run on long SDS-PAGE gels to resolve the 33- and 34-kDa CDK2 bands.