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. 2000 Sep;20(17):6300–6307. doi: 10.1128/mcb.20.17.6300-6307.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 2 — Association of p12^DOC-1 and CDK2 in cells. (A and B) CDK2 and p12^DOC-1 immunoblots showing the coprecipitation of p12^DOC-1 with CDK2 in 293 cells cotransfected with pCDK2 and pFLAG-DOC-1-wt or pFLAG-DOC-1-A3. Lanes 1 and 5, lysate (30 μg); lanes 2 and 6, immunoprecipitation using anti-FLAG monoclonal antibody (M5; Sigma Chemicals); lanes 3 and 7, CDK2 immunoprecipitation using anti-CDK2 monoclonal antibody (C18520 Clone 55; Transduction Laboratories); lanes 4 and 8, negative control using nonimmune mouse immunoglobulin G for immunoprecipitation. (C and D) Reprobing of the same membranes shown in panels A and B for cyclins A and E, respectively.