FIG. 4.
Amino acids 109 to 111 are necessary for p12DOC-1's association with CDK2. (A) Schematic of the mutagenesis strategy. Mutants were created by the Stratagene QuickChange site-directed mutagenesis system. The gray box in the C terminus (amino acids 62 to 115) is a domain that is homologous to a domain in a C. elegans protein, Y43F4B.7, and a related protein, DOC-1R (15). (B) In vitro association of the A1, A2, and A3 mutants with monomeric CDK2 in 293 cell lysate. One microgram of GST or GST-p12DOC-1 wild-type or mutant protein was mixed with 200 μg of 293 cell lysate. The CDK2 immunoblot was done using anti-CDK2 antibody (C18520 Clone 55; Transduction Laboratories).