FIG. 7.
Effect of ectopic expression of p12DOC-1 on CDK2-mediated phenotypes in 293 cells. (A) Immunodetection of FLAG-p12DOC-1 expression in 293 cells. (B) Effect of p12DOC-1 ectopic expression on cell growth at 12, 24, and 48 h posttransfection. (C) Cell cycle positions at 48 h posttransfection. (D) Tritiated thymidine incorporation at 48 h posttransfection. For panels A and B, 293 cells were transfected with pFLAG vector or pFLAG-DOC-1 for the indicated time points and analyzed. For panels C and D, 293 cells were cotransfected with a neomycin expression vector (pcDNA3) and selected for 2 weeks in the presence of G418 at 400 μg/ml. Data are from three independent experiments. Transfection efficiency of 293 cells with Lipofectamine Plus (Life Technologies/Gibco-BRL, Grand Island, N.Y.) is ∼70%.