| Antibody |
anti-GFP directly coupled to Alexa Fluor 488 (rabbit polyclonal) |
Invitrogen |
Cat # A21311 |
(1:400) |
| Chemical compound, drug |
CellMask Deep Red Plasma Membrane Stain |
Thermo Fisher Scientific |
Cat# C10046
|
(1:1000) |
| Chemical compound, drug |
Alexa Fluor 647 phalloidin |
Thermo Fisher Scientific |
Cat# A22287 |
(1:100) |
| Chemical compound, drug |
Alexa Fluor 488 phalloidin |
Thermo Fisher Scientific |
Cat# A12379 |
(1:200) |
| Chemical compound, drug |
Schneider’s Drosophila medium |
Thermo Fisher Scientific |
Cat# 21720-024 |
|
| Chemical compound, drug |
Fetal bovine serum |
Gibco |
Cat# 10438-018 |
|
| Chemical compound, drug |
Recombinant Human Insulin |
Millipore Sigma |
Cat# 12,643 |
|
| Other |
Soda Lime Glass Beads, 48–51 µm |
Cospheric LLC |
Cat# S-SLGMS-2.5 |
|
| Chemical compound, drug |
Formaldehyde, 16%, methanol free, Ultra-Pure |
Polysciences |
Cat# 18814-10 |
|
| Chemical compound, drug |
SlowFade Diamond Antifade mounting medium |
Invitrogen |
Cat# S36972
|
|
| Chemical compound, drug |
SlowFade Antifade Kit |
Thermo Fisher Scientific |
Cat# S2828 |
|
| Chemical compound, drug |
CK-666 |
Millipore Sigma |
Cat# SML0006 |
|
| Software, algorithm |
ImageJ version 2.1.0/1.53c |
|
https://fiji.sc/
|
|
| Software, algorithm |
Handbrake 1.3.3 The open source video transcoder |
HandBrake Team |
https://handbrake.fr/
|
|
| Software, algorithm |
Zen Blue |
Zeiss |
|
|
| Software, algorithm |
Zen Black |
Zeiss |
|
|
| Software, algorithm |
MetaMorph |
Molecular Devices |
|
|
| Software, algorithm |
Prism Version 8 |
Graphpad |
|
|
| Genetic reagent (D. melanogaster) |
w[1118] |
Bloomington Drosophila Stock Center |
BDSC: 3605; FlyBase ID: FBst0003605; RRID: BDSC_3605
|
FlyBase symbol: w[1,118] |
| Genetic reagent (D. melanogaster) |
sqh-GFP |
Vienna Drosophila Resource Center |
VDRC: 318484;FlyBaseID: FBst0492100 |
FlyBase symbol:PBac{fTRG00600.sfGFP-TVPTBF}VK00033 |
| Genetic reagent (D. melanogaster) |
MRLC-mCh |
Laboratory of Eric Wieschaus (Martin et al., 2009) |
|
sqhAx3/FM7;; sqh> sqh-mCh/TM3, Ser, actGFP |
| Genetic reagent (D. melanogaster) |
UAS-Pax-GFP |
Laboratory of Denise Montell (He et al., 2010) |
|
|
| Genetic reagent (D. melanogaster) |
daughterless-Gal4 |
Bloomington Drosophila Stock Center |
BDSC: 55851;FlyBaseID: FBst0055851; RRID:BDSC_55851
|
FlyBase symbol: w*; P{GAL4-da.G32}UH1, Sb1/TM6B, Tb1 |
| Genetic reagent (D. melanogaster) |
hs-FLP |
Bloomington Drosophila Stock Center |
BDSC: 8862; FlyBase ID: FBst0008862;RRID:BDSC_8862
|
FlyBase symbol: P{ry[+ t7.2] = hsFLP}22, w[*]} |
| Genetic reagent (D. melanogaster) |
act5c >> Gal4 |
Bloomington Drosophila Stock Center |
BDSC: 4780; FlyBase ID: FBst0004780;RRID:BDSC_4780
|
FlyBase symbol: y1 w*; P{GAL4-Act5C(FRT.CD2).P}S |
| Genetic reagent (D. melanogaster) |
UAS-Ftractin-Tom |
Bloomington Drosophila Stock Center |
BDSC: 58989;FlyBaseID: FBtp0095457;RRID:BDSC_4780
|
P{UASp-F-Tractin.tdTomato}15 A/SM6b; MKRS/TM2 |
| Genetic reagent (D. melanogaster) |
UAS-Lifeact-GFP |
Bloomington Drosophila Stock Center |
BDSC: 35544;FlyBaseID:FBst0035544; RRID:BDSC_35544
|
FlyBase symbol: y1 w*; P{UAS-Lifeact-GFP}VIE-260B
|
| Genetic reagent (D. melanogaster) |
UASMoesinABD-mCh 42c |
Laboratory of Brooke McCartney |
|
|
| Genetic reagent (D. melanogaster) |
UAS-Utrophin-ABD-GFP |
Laboratory of Thomas Lecuit (Rauzi et al., 2010) |
|
|
| Genetic reagent (D. melanogaster) |
Pax-GFP |
Kyoto Stock Center |
DGRC: 109971; FlyBaseID:FBst0325098 |
FlyBase symbol: w[1,118]; PBac{EGFP-IV}Pax[KM0601] |
| Genetic reagent (D. melanogaster) |
Talin-GFP |
Laboratory of Hugo Bellen (Venken et al., 2011) |
BDSC: 39649; FlyBaseID:FBst0039649;RRID:BDSC_39649
|
FlyBase symbol: y1 w*; Mi{PT-GFSTF.0}rheaMI00296-GFSTF.0 lncRNA:CR43910MI00296-GFSTF.0-X |
| Genetic reagent (D. melanogaster) |
ß-PS integrin-GFP |
Laboratory of Nicholas Brown (Klapholz et al., 2015) |
|
|
| Genetic reagent (D. melanogaster) |
traffic jam-Gal4 |
Kyoto Stock Center |
DGRC: 104055;FlyBaseID: FBst0302922 |
FlyBase symbol: y* w*; P{w + mW.hs=GawB}NP1624/ CyO, P{w-=UASlacZ. UW14}UW14 |
| Genetic reagent (D. melanogaster) |
UAS-Abi RNAi |
National Institute of Genetics, Japan |
NIG: 9749 R-3 |
|
| Genetic reagent (D. melanogaster) |
UAS-Sra1 RNAi |
Bloomington Drosophila Stock Center |
BDSC: 38294;FlyBaseID: FBst0038294; RRID:BDSC_38294
|
FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS01754}attP2 |
| Genetic reagent (D. melanogaster) |
act5c >> Gal4, UAS-RFP |
Bloomington Drosophila Stock Center |
BDSC: 30558; FlyBase ID: FBst0030558; RRID:BDSC_30558
|
FlyBase symbol: w[1,118]; P{w[+mC] = GAL4-Act5C(FRT.CD2).P}S, P{w[+mC] = UAS RFP.W}3/TM3, Sb (Ballestrem et al., 2001) |
| Genetic reagent (D. melanogaster) |
UAS-pTWFlag-C-DAAM |
Laboratory of József. Mihály (Matusek et al., 2006) |
|
|
| Genetic reagent (D. melanogaster) |
hsFLP RFP FRT 19A |
Bloomington Drosophila Stock Center |
BDSC: 31418;FlyBaseID: FBst0031418; RRID: BDSC_31418
|
FlyBase symbol: Ubi-mRFP.nls, w*, hsFLP neoFRT19A |
| Genetic reagent (D. melanogaster) |
DAAMA FRT 19A |
Bloomington Drosophila Stock Center |
BDSC: 52348;FlyBaseID: FBst0052348; RRID: BDSC_52348
|
FlyBase symbol: y1 DAAMA w* P{neoFRT}19 A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn+ |
| Genetic reagent (D. melanogaster) |
DAAMEx68
|
Laboratory of József. Mihály (Dollar et al., 2016) |
|
|
| Genetic reagent (D. melanogaster) |
19A FRT |
Bloomington Drosophila Stock Center |
BDSC: 1709;FlyBaseID: FBst0001709; RRID: BDSC_1709
|
FlyBase symbol: P{ry[+ t7.2] = neoFRT}19A; ry[506] |
| Genetic reagent (D. melanogaster) |
DAAMEx68 FRT 19A |
Recombination only, this study |
DAAMEx68 from J. Mihály; and 19A FRT from BDSC: 1,709 |
|
| Genetic reagent (D. melanogaster) |
RFP FRT 19A |
Bloomington Drosophila Stock Center |
BDSC: 31416; FlyBase ID:FBst0031416; RRID: BDSC_31416
|
FlyBase symbol: P{w[+ mC] = Ubi mRFP.nls}1, w[1,118], P{ry[+ t7.2] = neoFRT}19A |
| Genetic reagent (D. melanogaster) |
UAS-DAAM RNAi |
Vienna Drosophila Resource Center |
VDRC: 103921;FlyBase ID: FBst0475779 |
FlyBase symbol:P{KK102786}VIE-260B |
| Genetic reagent (D. melanogaster) |
UAS-Dia RNAi |
Vienna Drosophila Resource Center |
VDRC: 103914;FlyBase ID: FBst0475772 |
FlyBase symbol:P{KK101745}VIE-260B |
| Genetic reagent (D. melanogaster) |
UAS-Dia RNAi |
Vienna Drosophila Resource Center |
VDRC: 20518;FlyBase ID: FBst0453738 |
FlyBase symbol: w1118; P{GD9442}v20518 |
| Genetic reagent (D. melanogaster) |
UAS-FHOS RNAi |
Vienna Drosophila Resource Center |
VDRC: 45,838 (line has been discontinued) |
w[1,118]; +; P{GD10435}v145838 |
| Genetic reagent (D. melanogaster) |
UAS-FHOS RNAi |
Vienna Drosophila Resource Center |
VDRC: 34034; FlyBase ID: FBst0460422 |
FlyBase symbol: w1118; P{GD10435}v34034 |
| Genetic reagent (D. melanogaster) |
UAS-Frl RNAi |
Vienna Drosophila Resource Center |
VDRC: 34413;FlyBase ID: FBst0460614 |
FlyBase symbol: w1118; P{GD10799}v34413 |
| Genetic reagent (D. melanogaster) |
UAS-Frl RNAi |
Vienna Drosophila Resource Center |
VDRC: 110438; FlyBase ID: FBst0482010 |
FlyBase symbol:P{KK101703}VIE-260B |
| Genetic reagent (D. melanogaster) |
UAS-Capu RNAi |
Vienna Drosophila Resource Center |
VDRC: 110404; FlyBase ID: FBst0481976 |
FlyBase symbol: P{KK101400}VIE-260B |
| Genetic reagent (D. melanogaster) |
UAS-Capu RNAi |
Vienna Drosophila Resource Center |
VDRC: 34278; FlyBase ID: FBst0460552 |
FlyBase symbol: w1118; P{GD879}v34278 |
| Genetic reagent (D. melanogaster) |
UAS-Form3 RNAi |
Vienna Drosophila Resource Center |
VDRC: 45594; FlyBase ID: FBst0466234 |
FlyBase symbol: w1118; P{GD12856}v45594 |
| Genetic reagent (D. melanogaster) |
UAS-Form3 RNAi |
Vienna Drosophila Resource Center |
VDRC: 107473; FlyBase ID: FBst0479293 |
FlyBase symbol: P{KK110697}VIE-260B |
| Genetic reagent (D. melanogaster) |
UAS-Ena RNAi |
Vienna Drosophila Resource Center |
VDRC: 43058; FlyBase ID: FBst0464896 |
FlyBase symbol: w1118; P{GD8910}v43058/CyO |
| Genetic reagent (D. melanogaster) |
w[1118] DAAM-GFP |
Laboratory of József. Mihály (Molnár et al., 2014) |
|
|
| Genetic reagent (D. melanogaster) |
UAS-Dcr |
Bloomington Drosophila Stock Center |
BDSC: 24651; FlyBase ID: FBst0024651; RRID:BDSC_24651
|
FlyBase symbol: w1118; P{UAS-Dcr-2.D}10 |
| Genetic reagent (D. melanogaster) |
Cy2 Gal4 |
Laboratory of Nir Yakoby (Queenan et al., 1997) |
FlyBase ID: FBti0007266 |
FlyBase symbol: Dmel\P{GawB}CY2 |
