In situ hybridization of virus RNA in the eye structures. Detection of EBOV RNA by RNAScope ISH (red stain, arrows) in eye structures from experimentally inoculated ferrets. (a) Animal number 20016 (5.6 × 10−1 TCID50). Viral RNA within the iris and ciliary bodies is shown. Bar, 200 μm. (b) Animal number 01755 (5.6 × 10−1 TCID50). Viral RNA within the conjunctiva, mostly associated with blood vessels, is shown. Bar, 200 μm. (c) Animal number 20016 (5.6 × 10−1 TCID50). Viral RNA within the choroid is shown. Bar, 100 μm. (d) Animal number 01755 (5.6 × 10−1 TCID50). Viral RNA within the optic nerve is shown. Bar, 100 μm.