FIG 14.
Effect of BMS-806 on the synthesis, processing, and glycosylation of wild-type HIV-1AD8 Env. A549-Gag/Env cells were treated with BMS-806 (10 μM) or mock treated during doxycycline induction of Gag/Env expression. Lysates were prepared from cells (A) and supernatants containing virus-like particles (VLPs) (B) and were treated with peptide-N-glycosidase F (PNGase F) or endoglycosidase Hf (Endo Hf) or were mock treated (no Rx). The Envs were run on reducing SDS-polyacrylamide gels and analyzed by Western blotting. The deglycosylated gp160, gp120, and gp41 proteins (dgp160, dgp120, and dgp41, respectively) are indicated by arrows (red, PNGase F-treated sample; green, Endo Hf-treated sample). Data in this figure are representative of those obtained in two independent experiments.