TABLE 1.

Summary of HIV-1_JR-FL conformational states^a

Env	Source	Treatment	Occupancy of conformational state (%)			Reference
			State 1	State 2	State 3
Wild-type HIV-1JR-FL Env	Virion	None	50	26	24	42, 79
		BMS-806	55	18	27
HIV-1JR-FL Env(−)	Virion	None	25	42	33	79
		BMS-806	40	32	28
	Purified from cell membranes	BMS-806 + BS3 crosslinking	26	37	37	This study

^aThe relative occupancies (%) of conformational states for the indicated sources and treatments of HIV-1 Envs were derived from smFRET histograms. The FRET histograms were compiled from individual smFRET traces. The state distributions in the FRET histograms were fitted to the sum of three Gaussian distributions by hidden Markov modeling, and the occupancy of each state was obtained from the area under each Gaussian curve.