FIG 5.

Screening of entry inhibition potential of five drug-like compounds using pseudovirus assay. Screening entry inhibition potential of drug-like compounds in HEK-293T cells expressing human ACE2. (A–E) HEK-293T-hACE2 cells were pretreated with the indicated concentration of compounds and then inoculated with pseudotyped lentiviral particles expressing Spike glycoprotein of SARS-CoV-2. At 48 h postransduction, pseudotype entry was analyzed after normalization against untreated cells by determining luciferase activity in cell lysates. (F) The percentage inhibition of entry of pseudotyped lentiviral particles expressing Spike glycoprotein of SARS-CoV-2 was measured for MU-UNMC-2 at indicated concentration of the compounds (cells that received DMSO were considered vehicle controls). The IC₅₀ value was computed using four-parameter variable slope sigmoidal dose-response models using Graph Pad Prism 8.0 software.