FIG 8.

TLR4 deficiency impairs the proliferation of plasma cells after RABV immunization. (A to C) WT and TLR4^−/− mice (n = 5/group) were immunized i.m. with live RABV (1 × 10⁷ FFU/mouse) or DMEM. At 14 and 21 dpi, all mice were euthanized, and BM cells were collected from the femurs and tibias of the animals. (A) Gating strategies for detection of PCs (B220^low CD138⁺). (B) Representative flow cytometry plots of PCs. (C) Statistical results obtained for PCs. (D) Diagram of the various immune components and their roles in inducing the cDC2-Tfh-GC B axis. Data are presented as mean ± SD. Asterisks indicate significant differences between the groups; *, P < 0.05; ns, not significant.