FIG 7.
Effects of inhibitors on the entry of PDCoV into ST and LLC-PK1 cells. (A and B) ST (A) and LLC-PK1 (B) cells were pretreated with the highest concentration of chloroquine, NH4Cl, chlorpromazine, nystatin, EIPA, and wortmannin used in IPI-2I cells without resulting in obvious cytotoxic effects for 1 h at 37°C, respectively. Then, the cells were infected with PDCoV (MOI of 5) at 4°C for 1 h and then shifted to 37°C for 1 h (entry step). Cells were lysed to determine viral RNA copy numbers by RT-qPCR. The presented results represent the means and standard deviations of data from three independent experiments. **, P < 0.01.