TABLE 1.
Primers used for the construction of plasmids and real-time RT-PCR assay
| Primer | Nucleotide sequence |
|---|---|
| Dynamin-2-F | 5-GATAAGGAATTCGAGCTCATCGATATGGGCA-3 |
| Dynamin-2-R | 5-GCAGAGGGAAAAAGATCTGCTAGCCTAGTCG-3 |
| Caveolin-1-F | 5-ATAATCGATATGTCGGGGGGCAAATACGTAG-3 |
| Caveolin-1-R | 5-GCGCTCGAGTTATATTTCTTTCTGCATGTTGATGC-3 |
| Caveolin-1KSF-F | 5-GTGATCGATATGAAGAGTTTCCTGATTGAGATTC-3 |
| Caveolin-1DGI-F | 5-TTGATCGATATGGATGGCATCTGGAAGGCCAGCTT-3 |
| Rab5-F | 5-GATGGTACCATGGCTAATCGAGGAGCAACAAGAC-3 |
| Rab5-R | 5-GGAAGATCTTTAGTTACTACAACACTGACTCCTG-3 |
| Rab7-F | 5-GATGGTACCATGACCTCTAGGAAGAAAGTGTTGC-3 |
| Rab7-R | 5-GGAAGATCTTCAGCAACTGCAGCTTTCTGCCGAG-3 |
| Rab11-F | 5-GATGGTACCATGGGCACCCGCGACGACGAGTACG-3 |
| Rab11-R | 5-GGAAGATCTTTAGATGTTCTGACAGCACTGCACC-3 |
| qEPS15-F | 5-ACCATGATGGGATGCTTGACA-3 |
| qEPS15-R | 5-GGACACAACCCACGTTTTCC-3 |
| qCHC-F | 5-ACAAAGGTGGATAAATTAGACGC-3 |
| qCHC-R | 5-CCCATAGGGTGGTGCAGTAT-3 |
| qCaveolin 1-F | 5-TACGTAGACTCAGAGGGACATC-3 |
| qCaveolin 1-R | 5-GTGTGCGCGTCATACACTTG-3 |
| qDynamin-F | 5-TCCCCAACCAGGTGATCCG-3 |
| qDynamin-R | 5-AGCCCTTTTCCACATCACGG-3 |
| qRab5-F | 5-GTCGATTTCCAGGAAGCACA-3 |
| qRab5-R | 5-GCGTGGGTTCAGTAAGGTCTA-3 |
| qRab7-F | 5-ATCCTGGGAGATTCTGGAGTTG-3 |
| qRab7-R | 5-GTAGAAGGCCACGCCAAGAG-3 |
| qRab11-F | 5-ATTTGCGTCATCTCAGGGCA-3 |
| qRab11-R | 5-ACAATGCGGTATATCTCTGTCA-3 |
| qPDCoV-nsp16-F | 5-GCCCTCGGTGGTTCTATCTT-3 |
| qPDCoV-nsp16-R | 5-TCCTTAGCTTGCCCCAAATA-3 |
| qVSV-N-F | 5-ACGGCGTACTTCCAGATGG-3 |
| qVSV-N-R | 5-CTCGGTTCAAGATCCAGGT-3 |
| qSeV-HN-F | 5-AAAATTACATGGCTAGGAGGGAAAC-3 |
| qSeV-HN-R | 5-GTGATTGGAATGGTTGTGACTCTTA-3 |
| qGAPDH-F | 5-ACATGGCCTCCAAGGAGTAAGA-3 |
| qGAPDH-R | 5-GATCGAGTTGGGGCTGTGACT-3 |