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. 2021 Nov 16;2021:5319584. doi: 10.1155/2021/5319584

Figure 1.

Figure 1

Chemical structure of auraptene (AU) and the effects of AU on morphology and cell viability and on LTA-induced NO production and iNOS expression in RAW 264.7 cells. (a) Chemical structure of AU. (b), (c) Cells were pretreated with AU (5, 10, or 20 μM) for 24. Cell viabilities were determined by the MTT assay. Scale bar = 25 μm. (d), (e) Cells were untreated or pretreated with AU (5 and 10 μM) for 30 min prior to stimulation with LTA (5 μg/ml) for 24 h. Control cells were not treated with LTA or AU. NO was measured using the Griess reaction assay. iNOS expression was detected using western blotting assay. The values shown are the means ± S.E.M. of four independent experiments. ∗∗∗P < 0.001 vs. the control cells; ##P < 0.01 vs. LTA-stimulated cells.