Figure 5.

AU enhances antioxidant defense molecules in LTA-stimulated RAW cells. Cells were untreated or pretreated with AU (5 and 10 μM) for 30 min followed by LTA (5 μg/ml) for 24 h. The expression of HO-1 (a), catalase (CAT) activity (b), and glutathione (GSH) content (c) in LTA-induced RAW cells was determined using western blotting, native polyacrylamide gel electrophoresis (NATIVE-PAGE), and spectrophotometric analyses, respectively. The values shown are the means ± S.E.M. of four independent experiments. ^∗P < 0.05 vs. the control cells.